| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.079161 |
| Chromosome: | chromosome 14 |
| Location: | 3797102 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632550 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGTCCTGGCTTGAAGCTGCACCGTCGC |
| Internal bar code: | CGCAGATCCCAACGCGTCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 99.0506 |
| LEAP-Seq n confirming: | 939 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGGAAAGAGTGGGACAA |
| Suggested primer 2: | ACCGGTTTTGACATTGAAGC |