Insertion junction: LMJ.RY0402.079196_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.079196
Chromosome:chromosome 2
Location:2871366
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g094801 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAACCCACCGTGGGCCATCACCACCGCG
Internal bar code:TTACTTTTCGAGATCGCTTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:32
LEAP-Seq percent confirming:95.3052
LEAP-Seq n confirming:203
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAGACGGCGATTTCATCAAT
Suggested primer 2:TCGTACCGTTGATGCATTGT