Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.079203 |
Chromosome: | chromosome 17 |
Location: | 3929620 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g728450 | (1 of 1) IPR006626//IPR010308//IPR011050 - Parallel beta-helix repeat // TRP-like family // Pectin lyase fold/virulence factor | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGTACCGTAGGCTGCTGTCTAGCTGGG |
Internal bar code: | GAGGAATGACGGTAGTAGGGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 516 |
LEAP-Seq percent confirming: | 98.5866 |
LEAP-Seq n confirming: | 279 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGCCTAATGGAGGATTCAT |
Suggested primer 2: | CAACCTGCATGTTTGGTTTG |