| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079223 |
| Chromosome: | chromosome 3 |
| Location: | 1847479 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154651 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTCTGGCTGAATGGTAAAAGTGCCAGA |
| Internal bar code: | CTGCGTTGAGATTCGGCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 81.0596 |
| LEAP-Seq n confirming: | 2953 |
| LEAP-Seq n nonconfirming: | 690 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTGCTCAGTCAACTGCT |
| Suggested primer 2: | CTTGTCATTACTTGGGGCGT |