| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.079244 |
| Chromosome: | chromosome 5 |
| Location: | 3019415 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238500 | (1 of 4) K06941 - 23S rRNA (adenine2503-C2)-methyltransferase [EC:2.1.1.192] (rlmN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGACGCGTGGCAATGCTTCCCATCCCAC |
| Internal bar code: | ATGTGAGAGCCCGCTCGCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 670 |
| LEAP-Seq percent confirming: | 99.1165 |
| LEAP-Seq n confirming: | 3702 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCCCTACACTTCTGCTC |
| Suggested primer 2: | AATAATAGGGGCCGGACTTG |