| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079250 |
| Chromosome: | chromosome 16 |
| Location: | 1728421 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654900 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGATGGAGGGTACAGGGCAGAGGGACGGG |
| Internal bar code: | CGCCTTCAAGGACAGGTACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 988 |
| LEAP-Seq percent confirming: | 98.7705 |
| LEAP-Seq n confirming: | 1446 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTGCTCTACGTGGCTCG |
| Suggested primer 2: | AAAATCCATTTCCCGAAACC |