| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079293 |
| Chromosome: | chromosome 3 |
| Location: | 1559513 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152400 | EXN1 | (1 of 5) 3.1.13.5 - Ribonuclease D / RNase D; 3'-5' exonuclease | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCTGAAGCCGTCTGCGAGGTTCGTTGTC |
| Internal bar code: | GGGTCAGGACTACGGCACCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 99.0257 |
| LEAP-Seq n confirming: | 2236 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCACACACACATCTCGTT |
| Suggested primer 2: | AGATGCGACACATGCGTAAG |