Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.079314 |
Chromosome: | chromosome 4 |
Location: | 2496941 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g221950 | 5'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGCAACGCTGCAGCCCGTTTCAGACC |
Internal bar code: | GTGCACTCCGCCGTCGCATCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 510 |
LEAP-Seq percent confirming: | 98.9251 |
LEAP-Seq n confirming: | 2669 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTACTTCCCGTGAGAGCTGG |
Suggested primer 2: | CTGGGCATAAGGACCTTGAA |