| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079331 |
| Chromosome: | chromosome 16 |
| Location: | 4656412 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687450 | CPLD54 | (1 of 1) PTHR16254//PTHR16254:SF6 - POTASSIUM/PROTON ANTIPORTER-RELATED // K(+) EFFLUX ANTIPORTER 3, CHLOROPLASTIC; potassium ion transmembrane transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCACAGTGCTTCCTTGAAGCCTTCGGC |
| Internal bar code: | GGAGCTACAGTTTCTTCTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 97.846 |
| LEAP-Seq n confirming: | 2453 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAAGCTTCGTCACTGCCA |
| Suggested primer 2: | ATCCAAAACCGTTCTTGTCG |