| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079338 |
| Chromosome: | chromosome 2 |
| Location: | 3591805 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095137 | PFR1 | (1 of 1) 1.2.7.1 - Pyruvate synthase / Pyruvic-ferredoxin oxidoreductase; Pyruvate ferredoxin oxidoreductase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTTCCGCGGGCCTTCCTCGAGCGCCGCG |
| Internal bar code: | CCGGCCATGAGCGGATCCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.1614 |
| LEAP-Seq n confirming: | 2838 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTGGCACACGCTTTGTA |
| Suggested primer 2: | GTTGCATCAAGCTCCAGACA |