| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.079350 |
| Chromosome: | chromosome_12 |
| Location: | 2098216 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g509350 | FAP238 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGGATGAATGCCAAGCATGTCTGCTCCT |
| Internal bar code: | AACCCCGGGCGAGGAGGTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 90.7372 |
| LEAP-Seq n confirming: | 960 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACGACGCCACCATACAAA |
| Suggested primer 2: | CTGCTTCCTTAGCGTATGCC |