| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079354 |
| Chromosome: | chromosome 12 |
| Location: | 6196151 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536550 | ODA-IC1,IC78,ODA9,DIC1 | (1 of 1) K10409 - dynein intermediate chain 1, axonemal (DNAI1); Flagellar outer dynein arm intermediate chain 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGCAAGAAGGCGGCTGCGGCGGCAGC |
| Internal bar code: | TATGGTCCTTTGTTTACGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 99.5575 |
| LEAP-Seq n confirming: | 2025 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCAGTAGATCTGCCACA |
| Suggested primer 2: | CATAAGAGCTCGGATGAGGC |