| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079354 |
| Chromosome: | chromosome 12 |
| Location: | 6200577 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536600 | FAP78 | Flagellar Associated Protein 78; (1 of 7) PTHR11584:SF381 - PROTEIN PQN-80, ISOFORM B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGTCGCACTCCTTGACACGGTTCTTGA |
| Internal bar code: | TGTGGCGCACCTTAACTTCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 624 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGTAGGAGCTCACCAG |
| Suggested primer 2: | CACATGTACTACAACGGCGG |