Insertion junction: LMJ.RY0402.079388_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.079388
Chromosome:chromosome 3
Location:464459
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g145087 MTP1 Metal Transport Protein 1; (1 of 1) K14689 - solute carrier family 30 (zinc transporter), member 2 (SLC30A2, ZNT2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTGTGGTGTTACTCGACATCCCCCAATT
Internal bar code:TGTAGGAGGTATCTACCTAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:905
LEAP-Seq percent confirming:99.9106
LEAP-Seq n confirming:1117
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTTACCCCAAAACCCTCCAT
Suggested primer 2:GGTGGAACTCGTCACCCTAA