| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079432 |
| Chromosome: | chromosome 7 |
| Location: | 3855986 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338700 | (1 of 4) PF04676 - Protein similar to CwfJ C-terminus 2 (CwfJ_C_2) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGCTACCGCGCGTGCTTTGTTAGCAAA |
| Internal bar code: | CACCGGCCAGTCCTTCCGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 99.7798 |
| LEAP-Seq n confirming: | 2719 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGTATGCACCTAAGCG |
| Suggested primer 2: | ACGGAAAGCACGTTCAGACT |