| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079490 |
| Chromosome: | chromosome 13 |
| Location: | 2465275 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579950 | (1 of 1) PTHR12760:SF0 - ER MEMBRANE PROTEIN COMPLEX SUBUNIT 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCCTTCGTCCCTCACGCCTCCTCCCAC |
| Internal bar code: | ATTTAATATGACAATTCTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.4624 |
| LEAP-Seq n confirming: | 555 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTGCTACCACCGTAAAC |
| Suggested primer 2: | TGCCTACGCCTTCACTTCTT |