| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079500 |
| Chromosome: | chromosome 14 |
| Location: | 2646414 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626300 | (1 of 10) PF05347 - Complex 1 protein (LYR family) (Complex1_LYR) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCAGCATTCCGTGGCCTACAGGTATGC |
| Internal bar code: | AATAGCTTTGATGCGATGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 98.4848 |
| LEAP-Seq n confirming: | 260 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACATACCTCGGGAGCATA |
| Suggested primer 2: | GAGACAACGCGACAAATTGA |