| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079538 |
| Chromosome: | chromosome_10 |
| Location: | 1651713 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g429750 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCCGCCCGACTCCAGGTTGATGCGGATGT |
| Internal bar code: | AATGGCACGCATTACTTCCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 10 |
| LEAP-Seq percent confirming: | 43.369 |
| LEAP-Seq n confirming: | 1501 |
| LEAP-Seq n nonconfirming: | 1960 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTACCACCTGTCTGCAA |
| Suggested primer 2: | CGTGTGTGTGTGGTGTGTGT |