Insertion junction: LMJ.RY0402.079538_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.079538
Chromosome:chromosome_10
Location:1651784
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre10.g429750 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TAACTGGCGTTCTGGCGCAGCGGCACCGTA
Internal bar code:GATATCGTGGCCAGGCGCGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:168
LEAP-Seq percent confirming:98.7179
LEAP-Seq n confirming:539
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTACCACCTGTCTGCAA
Suggested primer 2:CGTGTGTGTGTGGTGTGTGT