| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079545 |
| Chromosome: | chromosome 8 |
| Location: | 4202342 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380300 | MSRA3 | (1 of 1) PTHR10173//PTHR10173:SF30 - METHIONINE SULFOXIDE REDUCTASE // SUBFAMILY NOT NAMED; Peptide methionine-S-sulfoxide reductase, msrA-type | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTCACCTTTGCCATCACCGCTTGCCAG |
| Internal bar code: | GGTTAGATAGTCGGAATTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 95.1515 |
| LEAP-Seq n confirming: | 20606 |
| LEAP-Seq n nonconfirming: | 1050 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGAGGAAACGGCATGAG |
| Suggested primer 2: | AAACTCCTGCCTACGGGAAT |