| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079555 |
| Chromosome: | chromosome 1 |
| Location: | 3788494 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024500 | (1 of 1) K16484 - rotatin (RTTN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCACCCACCGCGTCCATCTTGGCCGC |
| Internal bar code: | GAGACAGGTAGAGCCGTTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 81.9966 |
| LEAP-Seq n confirming: | 961 |
| LEAP-Seq n nonconfirming: | 211 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGAGGAAGCGATGGTAG |
| Suggested primer 2: | CAAGCAATAGGTATGGGCGT |