| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079562 |
| Chromosome: | chromosome 1 |
| Location: | 2652248 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015550 | DDA1 | (1 of 1) 4.1.2.52 - 4-hydroxy-2-oxoheptanedioate aldolase / HKHD aldolase; 2-dehydro-3-deoxyglucarate aldolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAAACCCACACGCGAGTACCCCATCGC |
| Internal bar code: | ATGGCCCCTATGTGGCAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 89 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTTTTAGGGTTCCCAAC |
| Suggested primer 2: | TGAGGAGAAGACGCATTGTG |