| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079583 |
| Chromosome: | chromosome 13 |
| Location: | 46719 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562150 | (1 of 1) PTHR23305:SF12 - GTP-BINDING PROTEIN-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGTTTTTAAATCTAGGTACTATGGCCA |
| Internal bar code: | GTTTTATGTTCGAGGCTAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1107 |
| LEAP-Seq percent confirming: | 99.644 |
| LEAP-Seq n confirming: | 12036 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCGCCATACTCATTGTTA |
| Suggested primer 2: | ATAAACTTCACACGGCGTCC |