| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079597 |
| Chromosome: | chromosome 12 |
| Location: | 3269087 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498200 | FAP128 | Flagellar Associated Protein 128 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCGTCGAGCGAACAAGAGCGTAAACCA |
| Internal bar code: | GGGACGGATGCGGTGGGTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 99.2243 |
| LEAP-Seq n confirming: | 1407 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACAGCTTGAAAGCACCC |
| Suggested primer 2: | ACGAGATGAGGGGTGTTACG |