| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.079603 |
| Chromosome: | chromosome 6 |
| Location: | 1546931 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260050 | (1 of 27) IPR015947 - PUA-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCCTCATGCTGCAGCGGCTGCGCCGT |
| Internal bar code: | TTGTCCCGCAGGAGACTCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 106 |
| LEAP-Seq percent confirming: | 95.082 |
| LEAP-Seq n confirming: | 232 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATCGACTGAGGAGTAGC |
| Suggested primer 2: | TCATTTGGTCGAGGAGCTTT |