| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079653 |
| Chromosome: | chromosome 6 |
| Location: | 2948356 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272950 | RPS18 | Ribosomal protein S18, component of cytosolic 80S ribosome and 4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTGCCCAAACAAAGCCTGCGGGCGCGA |
| Internal bar code: | TATTTCGGGCGCCCACTAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 36.9151 |
| LEAP-Seq n confirming: | 213 |
| LEAP-Seq n nonconfirming: | 364 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGATCACGAGGACTGTGC |
| Suggested primer 2: | GTCAACCTCAGCCTTCTTGC |