| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079656 |
| Chromosome: | chromosome 6 |
| Location: | 2908581 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272450 | OPR23,MBI1 | psbI mRNA stabilization factor; (1 of 19) PTHR21228//PTHR21228:SF20 - FAST LEU-RICH DOMAIN-CONTAINING // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGACATGCAGCTTTAGGGTGAGGGTGTAG |
| Internal bar code: | CGCTACAGGCCTGCTCTTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 99.6413 |
| LEAP-Seq n confirming: | 3333 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATCCCTGCGGATGTCAAG |
| Suggested primer 2: | TTTGCAGGATTGTCTGCTTG |