Insertion junction: LMJ.RY0402.079659_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.079659
Chromosome:chromosome 5
Location:2776943
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g237000 MAP1D Methionine aminopeptidase; (1 of 1) PTHR10804:SF82 - METHIONINE AMINOPEPTIDASE 1D, MITOCHONDRIAL intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCGACACGGATACCGCAGCCCCACCCA
Internal bar code:GCGGAGCTCGAGTCGCAGGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:413
LEAP-Seq percent confirming:99.2954
LEAP-Seq n confirming:1832
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AACCAGAGCCAACCATCAAC
Suggested primer 2:AACATCGACGTCACCGTGTA