| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.079672 |
| Chromosome: | chromosome 10 |
| Location: | 1492524 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428750 | (1 of 1) K14792 - rRNA biogenesis protein RRP5 (RRP5, PDCD11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCGTGATGGCGTCCGCCTCCTTGGTCA |
| Internal bar code: | TCCCGCAGCGTGGCGCTCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 69.3835 |
| LEAP-Seq n confirming: | 7721 |
| LEAP-Seq n nonconfirming: | 3407 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGAGGAGGGAGGTGTGT |
| Suggested primer 2: | GGAAGGACTACGTGGAGCAG |