| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079681 |
| Chromosome: | chromosome 17 |
| Location: | 383169 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698700 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGATGTGAGCAGCAGTGGCATAGGAA |
| Internal bar code: | AAGCGGGAGGGCTTTTAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 243 |
| LEAP-Seq percent confirming: | 71.6342 |
| LEAP-Seq n confirming: | 1692 |
| LEAP-Seq n nonconfirming: | 670 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATGCAGCTACTGCTGGT |
| Suggested primer 2: | ATTGGCGTTAGTCGAAGTGG |