| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079719 |
| Chromosome: | chromosome 8 |
| Location: | 1146811 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362700 | SMM30 | (1 of 8) 3.4.24.71 - Endothelin-converting enzyme 1 / ECE-1; S-adenosyl-L-methionine-dependent methyltransferase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCCTGGATTTATCACATGGCTACCTAG |
| Internal bar code: | GAGGGTGCCAACATTGGCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 488 |
| LEAP-Seq percent confirming: | 99.3399 |
| LEAP-Seq n confirming: | 1505 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTCTCCTACTTCCTCCT |
| Suggested primer 2: | AGTGTGCAAATGCTTTGTCG |