| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079729 |
| Chromosome: | chromosome 16 |
| Location: | 1502717 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653050 | (1 of 3) 2.1.1.200 - tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase / TrMet(Xm32) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTACCCTGGCCCTGACGCCGTTCGTTGCC |
| Internal bar code: | TCGGTCAGGCCGATGCTTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 99.9159 |
| LEAP-Seq n confirming: | 2377 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGTAGGGCCTGATTGGT |
| Suggested primer 2: | CACAGTCCACGATATGTGCC |