| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.079733 |
| Chromosome: | chromosome 6 |
| Location: | 2777928 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271050 | URN1 | (1 of 1) 3.2.2.3 - Uridine nucleosidase / Uridine ribohydrolase; Inosine/uridine-preferring nucleoside hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGGATACTGGTGAGTGGCTGTCGCAGC |
| Internal bar code: | ACCCTGTGGCGCCTGCGAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 98.9962 |
| LEAP-Seq n confirming: | 789 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTGAGTTCATTGTGCGT |
| Suggested primer 2: | GTTGGGTCGTGCAGGTAGAT |