| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079750 |
| Chromosome: | chromosome 4 |
| Location: | 1942390 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218300 | (1 of 1) K14774 - U3 small nucleolar RNA-associated protein 25 (UTP25, DEF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTCCGGCACTCCCACACCAAGCTCTCA |
| Internal bar code: | TAGACTCGGACTTGCATTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATGCCCGTACACTCCTT |
| Suggested primer 2: | TGAAAGGTTTTGGGGAGTTG |