| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079763 |
| Chromosome: | chromosome 12 |
| Location: | 8468360 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548201 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCTGCGGGCTGTGACAAGCTGTCGCAG |
| Internal bar code: | CCGGTAGGAGCGTTCCCCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 98.609 |
| LEAP-Seq n confirming: | 3261 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAAGCTCAATTCCAACA |
| Suggested primer 2: | TATCGTTTGGTGTCCGTTCA |