Insertion junction: LMJ.RY0402.079765_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.079765
Chromosome:chromosome 1
Location:6075717
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g043250 (1 of 3) PTHR30570:SF0 - PHOSPHATE-BINDING PROTEIN PSTS 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCCCTGAAGCCATACGCCGCTGTGTC
Internal bar code:GAACCGGTGTCACATGCCTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:615
LEAP-Seq percent confirming:97.3274
LEAP-Seq n confirming:874
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGAACCATGCACTCAAGA
Suggested primer 2:ACAGGACAGGACAGGACCAC