| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079771 |
| Chromosome: | chromosome 17 |
| Location: | 4189828 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730100 | RSE1,RSEP1 | (1 of 3) 3.4.24.85 - S2P endopeptidase / Sterol-regulatory element-binding proteins intramembrane protease; Intramembrane metalloprotease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCACGCACTCTTCAAAATTTCTTCAGG |
| Internal bar code: | GCACAGCGTAGACCGCGATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 95.2874 |
| LEAP-Seq n confirming: | 829 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCAGGAGGGAACCATCT |
| Suggested primer 2: | GGTGCTGGGAAATAACAGGA |