| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.079791 |
| Chromosome: | chromosome 8 |
| Location: | 4539591 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382700 | UBQ3 | (1 of 4) K08770 - ubiquitin C (UBC); Bi-ubiquitin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCGAGCTCAAGGAGTGCTCAACATTGT |
| Internal bar code: | CATCCCAGCGATCGTGAATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 394 |
| LEAP-Seq percent confirming: | 99.8162 |
| LEAP-Seq n confirming: | 1086 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTCAGAGGTAACGGTGC |
| Suggested primer 2: | GTAAATACCGGCAAGTGCGT |