Insertion junction: LMJ.RY0402.079802_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.079802
Chromosome:chromosome_7
Location:638192
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g316992 sense 3'UTR|outside_mRNA

Insertion site details

Flanking sequence (orientation from cassette outwards):ACCTGAAATAGCTGTGCACTAACGTGCATT
Internal bar code:CGGGCGTCAGGCCAAGGGATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:601
LEAP-Seq percent confirming:99.0946
LEAP-Seq n confirming:2189
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCAGAAGCACGCAATACT
Suggested primer 2:GGCGTTTGTCCAGAAAACAT