| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079804 |
| Chromosome: | chromosome 14 |
| Location: | 412385 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610647 | (1 of 3) PF02209 - Villin headpiece domain (VHP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAGCCTGCCCGCACAGGCAGTAGAGGC |
| Internal bar code: | AACTTGGAGCCACCATTCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCAGTACTTGCGACCCG |
| Suggested primer 2: | GAGGCAAGAGGATATGCGAG |