| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.079848 |
| Chromosome: | chromosome 14 |
| Location: | 412379 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610647 | (1 of 3) PF02209 - Villin headpiece domain (VHP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGAGGGGCAGGCCAGCAGACACGACAC |
| Internal bar code: | CGATGCTTGTGCACGAATGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 425 |
| LEAP-Seq percent confirming: | 95.6593 |
| LEAP-Seq n confirming: | 2909 |
| LEAP-Seq n nonconfirming: | 132 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCAGTACTTGCGACCCG |
| Suggested primer 2: | GAGGCAAGAGGATATGCGAG |