| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079909 |
| Chromosome: | scaffold 35 |
| Location: | 31511 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre35.g759447 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAAGGCACATCAGCTAGTACGCGACGTC |
| Internal bar code: | TTTACCCTTAGTGCAAGTACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 99.5215 |
| LEAP-Seq n confirming: | 624 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACCTCTCCTTCCCCTAC |
| Suggested primer 2: | CTGTTGCTGTTGCTGTTGCT |