| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.079947 |
| Chromosome: | chromosome_2 |
| Location: | 5990150 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g112200 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGGACCTGCCCGCCACGCGGCTGACGTTC |
| Internal bar code: | CGCACGTAGGGTTAAAGGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 99.114 |
| LEAP-Seq n confirming: | 4363 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTTCTGTTCGTGGACCT |
| Suggested primer 2: | GTCTGACGAGAAGTCCAGGC |