| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079947 |
| Chromosome: | chromosome 2 |
| Location: | 6021312 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112400 | CHI1 | (1 of 1) K12310 - Di-N-acetylchitobiase (CTBS); Chitinase-like hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTTGGCAAGGCCCTGTGTATTGTGTAAC |
| Internal bar code: | TTAACGTAATACATTTTGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 203 |
| LEAP-Seq percent confirming: | 98.1755 |
| LEAP-Seq n confirming: | 1130 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGCAAACTGCTGGTGAA |
| Suggested primer 2: | CAGCAACAGCACTATCGCAT |