| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.079975 |
| Chromosome: | chromosome_1 |
| Location: | 4309582 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029150 | DAU1,ODA7 | Outer row dynein assembly protein, predominantly cytoplasmic | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCACGCTCATGCAAGCCATTCTTCGCACTT |
| Internal bar code: | GCGTGGGTAGTAAAGAGTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 89.9536 |
| LEAP-Seq n confirming: | 582 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGACCTGTCGGTGATGT |
| Suggested primer 2: | AGGTAGCTTGGTTGAGGGGT |