| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.079993 |
| Chromosome: | chromosome 14 |
| Location: | 1785123 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619854 | ELG35 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 35 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACAGGCGTGTGTGCACATCCACACGCG |
| Internal bar code: | GAGCGGCGTGCACTAACCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 99.7134 |
| LEAP-Seq n confirming: | 6262 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTACGAGCAGTTCCTGAC |
| Suggested primer 2: | TAAGGAAGGGAAGGGAGGAA |