| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080000 |
| Chromosome: | chromosome 1 |
| Location: | 7933550 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g065092 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAACAATGATATGCGCCAAGGGACATGG |
| Internal bar code: | CCAGGAACTGGCAGCTGATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 98.5 |
| LEAP-Seq n confirming: | 2364 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCACCCAGAAACAGACC |
| Suggested primer 2: | ATACGGTCCAGAACGTCGTC |