Insertion junction: LMJ.RY0402.080017_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.080017
Chromosome:chromosome 11
Location:1439426
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467730 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase 3'UTR_intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGATGGCACAGAACGTGCAGAGCCTGCG
Internal bar code:GACGTCCATGATGCGGAGAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:613
LEAP-Seq percent confirming:93.2519
LEAP-Seq n confirming:1451
LEAP-Seq n nonconfirming:105
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AATACCGCCACCTTCAACTG
Suggested primer 2:CCTGTGACCTGGGAACACTT