| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.080038 |
| Chromosome: | chromosome 16 |
| Location: | 5371191 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681600 | CSV14 | Chlamydomonas-specific family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCATGATTATGGAGGAGGTCGCACCTA |
| Internal bar code: | CCGAACGCTTTGGGAACAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 97.9704 |
| LEAP-Seq n confirming: | 1786 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAGTTGCGGTCTGGAGT |
| Suggested primer 2: | AGACATGTAGGAGGTGCGCT |