| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080068 |
| Chromosome: | chromosome 3 |
| Location: | 3542344 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167778 | FAP382 | Flagellar Associated Protein 382 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTCTGCGGCTCGGACATAGTTGTGGT |
| Internal bar code: | AGGGCAGCGCGTTTCGGGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 96.9388 |
| LEAP-Seq n confirming: | 190 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCGTTTGGGTTTGAGT |
| Suggested primer 2: | TTGATGTTTGCTTCCTGCTG |